Introducing SALSA® Melt Assay
for Spinal Muscular Atrophy (SMA)

SALSA® MC002 SMA Newborn Screen (CE)

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. Popular applications include:
  • Predisposition to Cancer
  • Neuromuscular Disorders
  • Intellectual Disability
  • Solid Tumours


▷ Reduced sloping with the new MLPA buffer
Starting March 2019, all shipments of SALSA MLPA Reagent Kits will include a modified SALSA MLPA Buffer. There will also be a small change in the storage buffer of the SALSA Ligase-65 enzyme. The new reagents strongly reduce peak pattern sloping that is frequently observed when DNA is extracted with a magnetic beads-based system. If you did not have this issue, the new reagents will not affect your results.

If you previously had sloping caused by a magnetic bead-based DNA extraction method and used the S4 Sample Stabiliser to improve your results, you most likely do not need to add the S4 Sample Stabiliser anymore. The new reagents will not eliminate other causes of sloping.

More information on the background of this change, its effects, the timeline and our validation study can be found on this page.

▷ Launch of SALSA Melt Assay: SALSA MC002 SMA Newborn Screen
The SALSA MC002 SMA Newborn Screen is the latest product from MRC Holland, and the only product targeted at newborn population screening for a disorder. The assay is simple to use and runs on standard equipment allowing easy implementation and streamlining SMA screening. For more information visit our launch page, or see our press release.

MLPA Workshops
MLPA workshop figure

To increase your understanding of MLPA, MRC-Holland continues to organize one-day MLPA workshops. During the theoretical and practical sessions, participants learn how to get the most out of their MLPA data from setting up the experiment to the result interpretation and troubleshooting.

Check out our destinations for upcoming workshops on our Support Portal!

Support Portal
Do you have a technical question about MLPA or Coffalyser.Net? Visit our Support Portal for support-related content such as:
  • troubleshooting guidance;
  • knowledgebase articles (FAQ);
  • instruction videos;
  • e-learning modules;
  • manuals & other downloads.

You can also contact us directly from the portal, and optionally view and manage your correspondence with our Technical Support department.

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Improved Products
Breast and ovarian cancer, hereditary (HBOC)
P239-BRCA1 region
Breast cancer
Phelan-Mcdermid syndrome
Breast and ovarian cancer, hereditary (HBOC); Susceptibility to breast cancer; Susceptibility to other cancer types
Tumours, solid
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