SALSA MLPA P476 ZNRF3 probemix

application: Adrenocortical tumours and other solid tumours
region: ZNRF3, 22q12.1
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version: A1
sold since: 2018-07-02

item no. description price
P476-025R SALSA MLPA P476 ZNRF3 probemix – 25 rxn € 237
P476-050R SALSA MLPA P476 ZNRF3 probemix – 50 rxn € 474
P476-100R SALSA MLPA P476 ZNRF3 probemix – 100 rxn € 948
EK1-FAM SALSA MLPA EK1 reagent kit – 100 rxn - FAM € 294
EK1-Cy5 SALSA MLPA EK1 reagent kit – 100 rxn - Cy5 € 294
EK5-FAM SALSA MLPA EK5 reagent kit – 500 rxn - FAM € 1355
EK5-Cy5 SALSA MLPA EK5 reagent kit – 500 rxn - Cy5 € 1355

Please note that both a probemix and reagent kit are needed to perform MLPA.

General information: The SALSA MLPA Probemix P476 ZNRF3 is a research use only (RUO) assay for the detection of deletions and duplications in the ZNRF3 gene.

ZNRF3 is an E3 ubiquitin-protein ligase that acts as a negative feedback regulator of Wnt signalling (Hao et al. 2012). Three independent studies show homozygous deletions of the ZNRF3 gene in 10 to 16% of adrenocortical carcinoma cases (Assié et al. 2014; Juhlin et al. 2015; Zheng et al. 2016). Moreover, in 51% of microsatellite stable colorectal cancers deletion events at the ZNRF3 locus are detected (Bond et al. 2016). Deletions of the ZNRF3 gene are also found in osteoblastoma and prostate cancer (Nord et al. 2013; Robinson et al. 2015).

Probemix content: The SALSA MLPA Probemix P476-A1 ZNRF3 contains 42 MLPA probes with amplification products between 130 and 433 nucleotides (nt). This includes 15 probes for the ZNRF3 gene, plus four flanking probes for upstream genes and five flanking probes for downstream genes. In addition, 18 reference probes are included and target relatively copy number stable regions in various cancer types including adrenocortical carcinoma, colorectal cancer and prostate adenocarcinoma. Complete probe sequences and the identity of the genes detected by the reference probes is available in Table 2b of the product description and online (

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity Fragments (Q-fragments), two DNA Denaturation Fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at

product history
version A1: changes not specified
new products
Various cancer types
P480-WHS & Achondroplasia
Wolf Hirschhorn Syndrome, Achondroplasia
Meningioma, Coffin-Siris syndrome
P451-Chromosome 16
Breast cancer + various tumour types
improved products
Breast cancer susceptibility
Thyroid dysgenesis
P098-Wilson disease
Wilson disease
Thalassemia, beta-zero
Mismatch repair genes (MMR)
P253-NB mix 3
P252-NB mix 2
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