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SALSA MLPA Probemix P128 CYP450

Cytochrome P450

Region: CYP2D6, CYP2C9, CYP2C19, CYP1B1, CYP3A4, CYP3A5, CYP2E1, CYP1A1, CYP1A2, CYP2A6, CYP2B6, GSTP1, GSTT1 and GSTM1

This product is for basic research and intended for experienced users only! Interpretation of results may be complicated, and MRC Holland may only be able to provide basic support.

General information
The SALSA MLPA Probemix P128 CYP450 is a research use only (RUO) assay for the detection of deletions or duplications in the GSTM1, CYP1B1, CYP3A4, CYP3A5, CYP2C19, CYP2C9, CYP2E1, GSTP1, CYP1A2, CYP1A1, CYP2A6, CYP2B6, GSTT1 and CYP2D6 genes.
Cytochrome P450 enzymes are important in the biosynthesis and degradation of endogenous compounds such as steroids, lipids and vitamins. These enzymes reduce or alter the pharmacologic activity of many drugs and facilitate their elimination. Differences in drug metabolism in the intestine or liver between patients are major contributors to drug response, including adverse effects.
Glutathione transferases are a family of proteins that play an important role in detoxification by catalyzing the conjugation of many compounds with reduced glutathione.
In the normal population several of the targeted genes differ in copy number. More information per gene is given under Table 2.

The GSTM1 gene (8 exons), spans ~5.9 kb of genomic DNA and is located on 1p13.3, 110 Mb from the p-telomere. The CYP1B1 gene (3 exons), spans ~8.6 kb of genomic DNA and is located on 2p22.2, 38 Mb from p-telomere. The CYP3A4 gene (13 exons), spans ~27 kb of genomic DNA and is located on 7q22.1, 99 Mb from the p-telomere. The CYP3A5 gene (13 exons), spans ~32 kb of genomic DNA and is located on 7q22.1, 99 Mb from the p-telomere. The CYP2C19 gene (9 exons), spans ~93 kb of genomic DNA and is located on 10q23.33, 97 Mb from the p-telomere. The CYP2C9 gene (9 exons), spans ~51 kb of genomic DNA and is located on 10q23.33, 97 Mb from the p-telomere. The CYP2E1 gene (9 exons), spans ~12 kb of genomic DNA and is located on 10q26.3, 135 Mb from the p-telomere (close to the q-telomere). The GSTP1 gene (7 exons), spans ~2.8 kb of genomic DNA and is located on 11q13.2, 67 Mb from the p-telomere. The CYP1A2 gene (7 exons), spans ~7.8 kb of genomic DNA and is located on 15q24.1, 73 Mb from the p-telomere. The CYP1A1 gene (7 exons), spans ~6.0 kb of genomic DNA and is located on 15q24.1, 73 Mb from the p-telomere. The CYP2A6 gene (9 exons), spans ~6.9 kb of genomic DNA and is located on 19q13.2, 46 Mb from the p-telomere. The CYP2B6 gene (9 exons), spans ~27 kb of genomic DNA and is located on on 19q13.2, 46 Mb from the p-telomere. The GSTT1 gene (5 exon), spans ~8.2 kb of genomic DNA and is located on 22q11.23, 23 Mb from the p-telomere. The CYP2D6 gene (9 exons), spans ~4.3 kb of genomic DNA and is located on 22q13.2, 41 Mb from the p-telomere.
More information is available at https://www.ncbi.nlm.nih.gov/books/NBK1419/.

Probemix content
The SALSA MLPA Probemix P128-D2 CYP450 contains 52 MLPA probes with amplification products between 128 and 504 nucleotides (nt). At least two probes are present for each of the targeted genes. In addition, 12 reference probes are included that detect autosomal chromosomal locations. Complete probe sequences and the identity of the genes detected by the reference probes are available online (www.mrcholland.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mrcholland.com.

Order Items

Probemix

Item no.
Description
Technology
Price
P128-025R
SALSA MLPA Probemix P128 CYP450 – 25 rxn
€ 281.00 € 238.85
P128-050R
SALSA MLPA Probemix P128 CYP450 – 50 rxn
€ 550.00 € 467.50
P128-100R
SALSA MLPA Probemix P128 CYP450 – 100 rxn
€ 1075.00 € 913.75

Required Reagents (Sold Separately)

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM
€ 341.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5
€ 341.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM
€ 1571.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5
€ 1571.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM
€ 6037.00

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